Studies on the antigenic structure of the eye using the fluorescent antibody technique.
نویسنده
چکیده
THE cause of many cases of uveitis is unknown and it therefore seemed useful to examine a technique, little used in ophthalmic pathology, which might help to solve some problems. This method is the antigen-antibody technique of Coons and Kaplan (1950), who coupled fluorescein isocyanate to an antiserum with which they then treated fresh frozen sections of tissue. The site of the antigen-antibody reaction became visible as a green stain when the sections were examined with ultraviolet light. It is thus really a refined histological staining method. It has been used previously on the eye by Clayton (1954) and Clayton and Feldman (1955) to detect antigens of the embryonic lens, and by Witmer (1955) to study the intra-ocular production of antibody. Hill and Cruickshank (1953) and Cruickshank and Hill (1953) showed that an antiserum to rat glomeruli could be produced in rabbits, and by using the fluorescent antibody technique they showed that this antiserum reacted not only with the glomeruli, but also with basement membranes elsewhere in the body (spleen, lungs, lacrimal glands, blood-vessels, etc.). These workers did not investigate the reaction of their antiglomerular serum with eye tissues, and therefore the present investigations were made, in order to see whether basement membranes in the eye are similar in nature to those found elsewhere and also whether this technique could be successfully applied to the localization of antigen-antibody reactions in the eye.
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عنوان ژورنال:
- The British journal of ophthalmology
دوره 41 6 شماره
صفحات -
تاریخ انتشار 1957